Jo-1 antibodies

Jo-1 autoantibodies are also called histidyl-tRNA synthetase antibodies or polymyositis antibodies. They are an acknowledged marker for the disease polymyositis.

Testing for these autoantibodies is advisable when patients are demonstrating signs and symptoms compatible with connective tissue disease, such as muscle pain and limb weakness, Raynaud’s syndrome, arthritis, and concomitant pulmonary signs and symptoms.

The autoantigen responsible is the Jo-1 antigen, also called histidyl tRNA synthetase. The autoantigen is member of the amino acyl-tRNA synthetase family of enzymes and part of the ENA set. Histidyl tRNA synthetase is a cytoplasmic enzyme found in all nucleated cells. During protein biosynthesis histidyl tRNA synthetase catalyses the binding of the amino acid histidine to transfer RNA (tRNA). Jo-1 antibodies bind to conformational epitopes of the enzyme protein, inhibiting its catalytic activity.

Most commonly Jo-1 antibodies occur in myositis patients with interstitial lung disease. In patients with interstitial pulmonary fibrosis and symmetrical polyarthritis Jo-1 antibodies are detectable in up to 50% of cases.

 

Tests offered by ORGENTEC Diagnostika

ELISA/Alegria®:

ANA Detect (ORG 600, ORG 200)
ANAcombi (ORG 539)
ANAscreen (ORG 538, ORG 238)
Anti-Jo-1 (ORG 513, ORG 213)
ENA-6-Profile (ORG 546)
ENAcombi (ORG 514)
ENAscreen (ORG 506, ORG 206)

Immunoblot:

ANA-9-Line (ORG 710)
Myositis plus (ORG 760)
Nucleo-9-Line (ORG 711)

Indirect Immunofluorescence Assay (IFA):

Anti-Nuclear Antibodies HEp-2 (ANA-HEp-2) (ORG 870, ORG 800)

 

Advertisements

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s